LSBio
ZNF215 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF215 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF215 antibodies →
$725.00each
Catalog Number: LS-G114374-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF215 (NM_013250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BAZ2 | ZSCAN43 | Zinc finger protein 215 | ZKSCAN11 | ZNF215 | BAZ 2 | BAZ-2
Origin Species
Human
NCBI GenBank
NM_013250
SwissProt
Q9UL58

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPLSKLMAI SKPRNLSLRE QREVLRADMS WQQETNPVVE THDSEASRQK FRHFQYLKVS
61GPHEALSQLW ELCLQWLRPE IHTKKQIIEL LVLEQFLAIL PEEVRTWVNL QHPNNSKDMV
121TLIEDVIEML EDEDMPCKDS ALQMGSIKEK MKAGSRTGKP QEPVTFKDVV VEFSKEEWGQ
181LDSAVKNLYR NVMLENFRNL NSLRKAHLLS KPFESLKLES KKKRWIMEKE IPRKTIFDMK
241SISGEESSHG VIMTRLTESG HPSSDAWKGE NWLYRNQKKW DINLPQEAFI PETIYTEEED
301FECSENKKSF DINSVSSICA IQVGIPSRKG SPKCDKFKTY FKFNLDSVGK QHSEYEYGND
361LSLSTDIRHQ KSHTTMNSYE CYQCGKAFCR SSSLIRHQII HTGEKPYKCS ECGRFFNRRT
421NLTKHQKLHA EAKACTSNKC GKAFSKSEDS NNPTLHFGNN FYQCVNCGKS FNRSSSLIRH
481QMIHTGEKPF KCKECSKAFN RSSNLVKHQK LHTRDKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

517 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.