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ZNF212 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF212 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF212 antibodies →
$504.00each
Catalog Number: LS-G114546-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF212 (NM_012256), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2H2-150 | ZNF212 | ZNFC150 | Zinc finger protein 212 | Zinc finger protein C2H2-150
Origin Species
Human
NCBI GenBank
NM_012256
SwissProt
Q9UDV6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MAESAPARHR RKRRSTPLTS STLPSQATEK SSYFQTTEIS LWTVVAAIQA VEKKMESQAA
61RLQSLEGRTG TAEKKLADCE KMAVEFGNQL EGKWAVLGTL LQEYGLLQRR LENVENLLRN
121RNFWILRLPP GSKGEAPKVS RSLENDGVCF TEQEWENLED WQKELYRNVM ESNYETLVSL
181KVLGQTEGEA ELGTEMLGDL EEEGPGGAHP AGGVMIKQEL QYTQEGPADL PGEFSCIAEE
241QAFLSPEQTE LWGGQGSSVL LETGPGDSTL EEPVGSRVPS SSRTVGCPKQ KSHRQVQLDQ
301ECGQGLKLKK DTSRPYECSE CEITFRYKQQ LATHLRSHSG WGSCTPEEPE ESLRPRPRLK
361PQTKKAKLHQ CDVCLRSFSC KVSLVTHQRC HLQEGPSAGQ HVQERFSPNS LVALPGHIPW
421RKSRSSLICG YCGKSFSHPS DLVRHQRIHT GERPYSCTEC EKSFVQKQHL LQHQKIHQRE
481RGGLALEPGR PNGLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.