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ZNF205 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF205 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF205 antibodies →
$504.00each
Catalog Number: LS-G117322-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF205 (NM_003456), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF210 | Zfp13 | Zinc finger protein 205 | Zinc finger protein 210 | ZNF205 | RhitH
Origin Species
Human
NCBI GenBank
NM_003456
SwissProt
O95201

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSADGGGIQD TQDKETPPEV PDRGHPHQEM PSKLGEAVPS GDAQESLHIK MEPEEPHSEG
61ASQEDGAQGA WGWAPLSHGS KEKALFLPGG ALPSPRIPVL SREGRTRDRQ MAAALLTAWS
121QMPVTFEDVA LYLSREEWGR LDHTQQNFYR DVLQKKNGLS LGFPFSRPFW APQAHGKGEA
181SGSSRQAGDE KEWRGACTGA VEVGQRVQTS SVAALGNVKP FRTRAGRVQW GVPQCAQEAA
241CGRSSGPAKD SGQPAEPDRT PDAAPPDPSP TEPQEYRVPE KPNEEEKGAP ESGEEGLAPD
301SEVGRKSYRC EQCGKGFSWH SHLVTHRRTH TGEKPYACTD CGKRFGRSSH LIQHQIIHTG
361EKPYTCPACR KSFSHHSTLI QHQRIHTGEK PYVCDRCAKR FTRRSDLVTH QGTHTGAKPH
421KCPICAKCFT QSSALVTHQR THTGVKPYPC PECGKCFSQR SNLIAHNRTH TGEKPYHCLD
481CGKSFSHSSH LTAHQRTHRG VRPYACPLCG KSFSRRSNLH RHEKIHTTGP KALAMLMLGA
541AAAGALATPP PAPT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.