LSBio
ZNF202 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF202 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100053-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF202 (NM_003455), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 202 | ZNF202 | ZKSCAN10 | ZSCAN42
Origin Species
Human
NCBI GenBank
NM_003455
SwissProt
O95125

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATAVEPEDQ DLWEEEGILM VKLEDDFTCR PESVLQRDDP VLETSHQNFR RFRYQEAASP
61REALIRLREL CHQWLRPERR TKEQILELLV LEQFLTVLPG ELQSWVRGQR PESGEEAVTL
121VEGLQKQPRR PRRWVTVHVH GQEVLSEETV HLGAEPESPN ELQDPVQSST PEQSPEETTQ
181SPDLGAPAEQ RPHQEEELQT LQESEVPVPE DPDLPAERSS GDSEMVALLT ALSQGLVTFK
241DVAVCFSQDQ WSDLDPTQKE FYGEYVLEED CGIVVSLSFP IPRPDEISQV REEEPWVPDI
301QEPQETQEPE ILSFTYTGDR SKDEEECLEQ EDLSLEDIHR PVLGEPEIHQ TPDWEIVFED
361NPGRLNERRF GTNISQVNSF VNLRETTPVH PLLGRHHDCS VCGKSFTCNS HLVRHLRTHT
421GEKPYKCMEC GKSYTRSSHL ARHQKVHKMN APYKYPLNRK NLEETSPVTQ AERTPSVEKP
481YRCDDCGKHF RWTSDLVRHQ RTHTGEKPFF CTICGKSFSQ KSVLTTHQRI HLGGKPYLCG
541ECGEDFSEHR RYLAHRKTHA AEELYLCSEC GRCFTHSAAF AKHLRGHASV RPCRCNECGK
601SFSRRDHLVR HQRTHTGEKP FTCPTCGKSF SRGYHLIRHQ RTHSEKTS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

648 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.