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ZNF200 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF200 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126720-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP200(ZNF200) (NM_001145447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF200 | Zinc finger protein 200 | ZNFMF
Origin Species
Human
NCBI GenBank
NM_003454
SwissProt
P98182

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMAAKVVPMP PKPKQSFILR VPPDSKLGQD LLRDATNGPK TIHQLVLEHF LTFLPKPSLV
61QPSQKVKETL VIMKDVSSSL QNRVHPRPLV KLLPKGVQKE QETVSLYLKA NPEELVVFED
121LNVFHCQEEC VSLDPTQQLT SEKEDDSSVG EMMLLAVNGS NPEGEDPERE PVENEDYREK
181SSDDDEMDSS LVSQQPPDNQ EKERLNTSIP QKRKMRNLLV TIENDTPLEE LSKYVDISII
241ALTRNRRTRR WYTCPLCGKQ FNESSYLISH QRTHTGEKPY DCNHCGKSFN HKTNLNKHER
301IHTGEKPYSC SQCGKNFRQN SHRSRHEGIH IREKIFKCPE CGKTFPKNEE FVLHLQSHEA
361ERPYGCKKCG RRFGRLSNCT RHEKTHSACK TRKQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

395 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.