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ZNF20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF20 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF20 antibodies →
$504.00each
Catalog Number: LS-G110750-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF20 (NM_021143), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein KOX13 | Zinc finger protein 20 | KOX13 | ZNF20
Origin Species
Human
SwissProt
P17024

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMFQDSVAFE DVAVSFTQEE WALLDPSQKN LYRDVMQETF KNLTSVGKTW KVQNIEDEYK
61NPRRNLSLMR EKLCESKESH HCGESFNQIA DDMLNRKTLP GITPCESSVC GEVGTGHSSL
121NTHIRADTGH KSSEYQEYGE NPYRNKECKK AFSYLDSFQS HDKACTKEKP YDGKECTETF
181ISHSCIQRHR VMHSGDGPYK CKFCGKAFYF LNLCLIHERI HTGVKPYKCK QCGKAFTRST
241TLPVHERTHT GVNADECKEC GNAFSFPSEI RRHKRSHTGE KPYECKQCGK VFISFSSIQY
301HKMTHTGEKP YECKQCGKAF RCGSHLQKHG RTHTGEKPYE CRQCGKAFRC TSDLQRHEKT
361HTEDKPYGCK QCGKGFRCAS QLQIHERTHS GEKPHECKEC GKVFKYFSSL RIHERTHTGE
421KPHECKQCGK AFRYFSSLHI HERTHTGDKP YECKVCGKAF TCSSSIRYHE RTHTGEKPYE
481CKHCGKAFIS NYIRYHERTH TGEKPYQCKQ CGKAFIRASS CREHERTHTI NR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

532 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.