LSBio
ZNF185 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF185 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G130104-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF185 (NM_001178110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LIM domain protein ZNF185 | p1-A | ZNF185 | Zinc finger protein 185
Origin Species
Human
NCBI GenBank
NM_007150

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSISALGGRT KGKPLPPGEE ERNNVLKQMK VRTTLKGDKS WITKQDESEG RTIELPSGRS
61RATSFSSAGE VPKPRPPSTR APTGYIIRGV FTKPIDSSSQ PQQQFPKANG TPKSAASLVR
121TANAGPPRPS SSGYKMTTED YKKLAPYNIR RSSTSGDTEE EEEEEVVPFS SDEQKRRSEA
181ASGVLRRTAP REHSYVLSAA KKSTGPTQET QAPFIAKRVE VVEEDGPSEK SQDPPALARS
241TPGSNRSSPG NKDKEAPCSR ELQRDLAGEE AFRAPNTDAA RSSAQLSDGN VGSGATGSRP
301EGLAAVDIGS ERGSSSATSV SAVPADRKSN STAAQEDAKA DPKGALADYE GKDVATRVGE
361AWQERPGAPR GGQGDPAVPA QQPADPSTPE RQSSPSGSEQ LVRRESCGSS VLTDFEGKDV
421ATKVGEAWQD RPGAPRGGQG DPAVPTQQPA DPSTPEQQNS PSGSEQFVRR ESCTSRVRSP
481SSCMVTVTVT ATSEQPHIYI PAPASELDSS STTKGILFVK EYVNASEVSS GKPVSARYSN
541VSSIEDSFAM EKKPPCGSTP YSERTTGGIC TYCNREIRDC PKITLEHLGI CCHEYCFKCG
601ICSKPMGDLL DQIFIHRDTI HCGKCYEKLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

630 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.