LSBio
ZNF184 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF184 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114819-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF184 (NM_007149), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF184 | Zinc finger protein 184
Origin Species
Human
NCBI GenBank
U66561
SwissProt
Q99676

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDLSSPDST LLQGGHNLLS SASFQESVTF KDVIVDFTQE EWKQLDPGQR DLFRDVTLEN
61YTHLVSIGLQ VSKPDVISQL EQGTEPWIME PSIPVGTCAD WETRLENSVS APEPDISEEE
121LSPEVIVEKH KRDDSWSSNL LESWEYEGSL ERQQANQQTL PKEIKVTEKT IPSWEKGPVN
181NEFGKSVNVS SNLVTQEPSP EETSTKRSIK QNSNPVKKEK SCKCNECGKA FSYCSALIRH
241QRTHTGEKPY KCNECEKAFS RSENLINHQR IHTGDKPYKC DQCGKGFIEG PSLTQHQRIH
301TGEKPYKCDE CGKAFSQRTH LVQHQRIHTG EKPYTCNECG KAFSQRGHFM EHQKIHTGEK
361PFKCDECDKT FTRSTHLTQH QKIHTGEKTY KCNECGKAFN GPSTFIRHHM IHTGEKPYEC
421NECGKAFSQH SNLTQHQKTH TGEKPYDCAE CGKSFSYWSS LAQHLKIHTG EKPYKCNECG
481KAFSYCSSLT QHRRIHTREK PFECSECGKA FSYLSNLNQH QKTHTQEKAY ECKECGKAFI
541RSSSLAKHER IHTGEKPYQC HECGKTFSYG SSLIQHRKIH TGERPYKCNE CGRAFNQNIH
601LTQHKRIHTG AKPYECAECG KAFRHCSSLA QHQKTHTEEK PYQCNKCEKT FSQSSHLTQH
661QRIHTGEKPY KCNECDKAFS RSTHLTEHQN THTGEKPYNC NECRKTFSQS TYLIQHQRIH
721SGEKPFGCND CGKSFRYRSA LNKHQRLHPG I
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

751 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.