Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF182 / ZNF21 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122079-100
Catalog Number: LS-G122079
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF182 (NM_001007088), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KOX14 | HHZ150 | ZNF21 | Zinc finger protein 21 | ZNF182 | Zfp182 | Zinc finger protein 182 | Zinc finger protein KOX14
- Origin Species
- Human
- NCBI GenBank
- NM_006962
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKPQGLVTF EDVAVDFTQE EWQYLNPPQR TLYRDVMLET YSNLVFVGQQ VTKPNLILKL61EVEECPAEGK IPFWNFPEVC QVDEQIERQH QDDQDKCLLM QVGFSDKKTI ITKSARDCHE121FGNILHLSTN LVASIQRPDK HESFGNNMVD NLDLFSRSSA ENKYDNGCAK LFFHTEYEKT181NPGMKPYGYK ECGKGLRRKK GLSLHQRIKN GEKPFECTAC RKTFSKKSHL IVHWRTHTGE241KPFGCTECGK AFSQKSQLII HLRTHTGERP FECPECGKAF REKSTVIIHY RTHTGEKPYE301CNECGKAFTQ KSNLIVHQKT HTGEKTYECT KCGESFIQKL DLIIHHSTHT GKKPHECNEC361KKTFSDKSTL IIHQRTHTGE KPHKCTECGK SFNEKSTLIV HQRTHTGEKP YECDVCGKTF421TQKSNLGVHQ RTHSGEKPFE CNECEKAFSQ KSYLMLHQRG HTGEKPYECN ECEKAFSQKS481YLIIHQRTHT EEKPYKCNEC GKAFREKSKL IIHQRIHTGE KPYECPVCWK AFSQKSQLII541HQRTHTGEKP YACTECGKAF REKSTFTVHQ RTHTGEKPYK CTECGKAFTQ KSNLIVHQRT601HAGKKAHGRG HTRKSKFMAHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
620 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.