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ZNF180 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF180 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114381-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF180 (NM_013256), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HHZ168 | Zinc finger protein 180 | ZNF180
Origin Species
Human
NCBI GenBank
NM_013256
SwissProt
Q9UJW8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRVYAGSWK RCAAQDLSTL LCLEESMEEQ DEKPPEPPKA CAQDSFLPQE IIIKVEGEDT
61GSLTIPSQEG VNFKIVTVDF TREEQGTWNP AQRTLDRDVI LENHRDLVSW DLATAVGKKD
121STSKQRIFDE EPANGVKIER FTRDDPWLSS CEEVDDCKDQ LEKQQEKQEI LLQEVAFTQR
181KAVIHERVCK SDETGEKSGL NSSLFSSPVI PIRNHFHKHV SHAKKWHLNA AVNSHQKINE
241NETLYENNEC GKPPQSIHLI QFTRTQTKDK CYGFSDRIQS FCHGTPLHIH EKIHGGGKTF
301DFKECGQVLN PKISHNEQQR IPFEESQYKC SETSHSSSLT QNMRNNSEEK PFECNQCGKS
361FSWSSHLVAH QRTHTGEKPY ECSECGKSFS RSSHLVSHQR THTGEKPYRC NQCGKSFSQS
421YVLVVHQRTH TGEKPYECNQ CGKSFRQSYK LIAHQRTHTG EKPYECNQCG KSFIQSYKLI
481AHQRIHTGEK PYECNQCGKS FSQSYKLVAH QRTHTGEKPF ECNQCGKSFS WSSQLVAHQR
541THTGEKPYEC SECGKSFNRS SHLVMHQRIH TGEKPYECNQ CGKSFSQSYV LVVHQRTHTG
601EKPYECSQCG KSFRQSSCLT QHQRTHTGEK PFECNQCGKT FSLSARLIVH QRTHTGEKPF
661TCIQCGKAFI NSYKLIRHQA THTEEKLYEC N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

691 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.