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ZNF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF18 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106930-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF18 (NM_144680), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 535 | Zinc finger protein KOX11 | ZKSCAN6 | ZNF18 | ZSCAN38 | Zfp535 | Zinc finger protein 18 | ZNF535 | HDSG1 | KOX11
Origin Species
Human
NCBI GenBank
NM_144680
SwissProt
P17022

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVDLGQALG LLPSLAKAED SQFSESDAAL QEELSSPETA RQLFRQFRYQ VMSGPHETLK
61QLRKLCFQWL QPEVHTKEQI LEILMLEQFL TILPGEIQMW VRKQCPGSGE EAVTLVESLK
121GDPQRLWQWI SIQVLGQDIL SEKMESPSCQ VGEVEPHLEV VPQELGLENS SSGPGELLSH
181IVKEESDTEA ELALAASQPA RLEERLIRDR DLGASLLPAA PQEQWRQLDS TQKEQYWDLI
241LETYGKMVSG AGISHPKSDL TNSIEFGEEL AGIYLHVNEK IPRPTCIGDR QENDKENLNL
301ENHRDQELLH ASCQASGEVP SQASLRGFFT EDEPGCFGEG ENLPEALQNI QDEGTGEQLS
361PQERISEKQL GQHLPNPHSG EMSTMWLEEK RETSQKGQPR APMAQKLPTC RECGKTFYRN
421SQLIFHQRTH TGETYFQCTI CKKAFLRSSD FVKHQRTHTG EKPCKCDYCG KGFSDFSGLR
481HHEKIHTGEK PYKCPICEKS FIQRSNFNRH QRVHTGEKPY KCSHCGKSFS WSSSLDKHQR
541SHLGKKPFQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

549 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.