LSBio
ZNF175 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF175 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF175 antibodies →
$504.00each
Catalog Number: LS-G114817-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF175 (NM_007147), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
OTK18 | Zinc finger protein OTK18 | ZNF175 | Zinc finger protein 175
Origin Species
Human
NCBI GenBank
NM_007147
SwissProt
Q9Y473

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPADVNLSQK PQVLGPEKQD GSCEASVSFE DVTVDFSREE WQQLDPAQRC LYRDVMLELY
61SHLFAVGYHI PNPEVIFRML KEKEPRVEEA EVSHQRCQER EFGLEIPQKE ISKKASFQKD
121MVGEFTRDGS WCSILEELRL DADRTKKDEQ NQIQPMSHSA FFNKKTLNTE SNCEYKDPGK
181MIRTRPHLAS SQKQPQKCCL FTESLKLNLE VNGQNESNDT EQLDDVVGSG QLFSHSSSDA
241CSKNIHTGET FCKGNQCRKV CGHKQSLKQH QIHTQKKPDG CSECGGSFTQ KSHLFAQQRI
301HSVGNLHECG KCGKAFMPQL KLSVYLTDHT GDIPCICKEC GKVFIQRSEL LTHQKTHTRK
361KPYKCHDCGK AFFQMLSLFR HQRTHSREKL YECSECGKGF SQNSTLIIHQ KIHTGERQYA
421CSECGKAFTQ KSTLSLHQRI HSGQKSYVCI ECGQAFIQKA HLIVHQRSHT GEKPYQCHNC
481GKSFISKSQL DIHHRIHTGE KPYECSDCGK TFTQKSHLNI HQKIHTGERH HVCSECGKAF
541NQKSILSMHQ RIHTGEKPYK CSECGKAFTS KSQFKEHQRI HTGEKPYVCT ECGKAFNGRS
601NFHKHQITHT RERPFVCYKC GKAFVQKSEL ITHQRTHMGE KPYECLDCGK SFSKKPQLKV
661HQRIHTGERP YVCSECGKAF NNRSNFNKHQ TTHTRDKSYK CSYSVKGFTK Q
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

711 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.