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ZNF155 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF155 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117328-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF155 (NM_003445), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF155 | PHZ-96 | Zinc finger protein 155 | KRAB A domain
Origin Species
Human
NCBI GenBank
NM_003445
SwissProt
Q12901

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTTFKEAVTF KDVAVVFTEE ELGLLDPAQR KLYRDVMLEN FRNLLSVGHQ PFHQDTCHFL
61REEKFWMMGT ATQREGNSGG KIQTELESVP EAGAHEEWSC QQIWEQIAKD LTRSQDSIIN
121NSQFFENGDV PSQVEAGLPT IHTGQKPSQG GKCKQSISDV PIFDLPQQLY SEEKSYTCDE
181CGKSICYISA LHVHQRVHVG EKLFMCDVCG KEFSQSSHLQ THQRVHTGEK PFKCEQCGKG
241FSRRSALNVH RKLHTGEKPY ICEACGKAFI HDSQLKEHKR IHTGEKPFKC DICGKTFYFR
301SRLKSHSMVH TGEKPFRCDT CDKSFHQRSA LNRHCMVHTG EKPYRCEQCG KGFIGRLDFY
361KHQVVHTGEK PYNCKECGKS FRWSSCLLNH QRVHSGEKSF KCEECGKGFY TNSQLSSHQR
421SHSGEKPYKC EECGKGYVTK FNLDLHQRVH TGERPYNCKE CGKNFSRASS ILNHKRLHCQ
481KKPFKCEDCG KRLVHRTYRK DQPRDYSGEN PSKCEDCGRR YKRRLNLDIL LSLFLNDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

538 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.