LSBio
ZNF142 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF142 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF142 antibodies →
$725.00each
Catalog Number: LS-G124135-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF142 (NM_001105537), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HA4654 | KIAA0236 | PHZ-49 | Zinc finger protein 142 | ZNF142
Origin Species
Human
NCBI GenBank
AB093218

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
187.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTDPLLDSQP ASSTGEMDGL CPELLLIPPP LSNRGILGPV QSPCPSRDPA PIPTEPGCLL
61VEATATEEGP GNMEIIVETV AGTLTPGAPG ETPAPKLPPG EREPSQEAGT PLPGQETAEE
121ENVEKEEKSD TQKDSQKAVD KGQGAQRLEG DVVSGTESLF KTHMCPECKR CFKKRTHLVE
181HLHLHFPDPS LQCPNCQKFF TSKSKLKTHL LRELGEKAHH CPLCHYSAVE RNALNRHMAS
241MHEDISNFYS DTYACPVCRE EFRLSQALKE HLKSHTAAAA AEPLPLRCFQ EGCSYAAPDR
301KAFIKHLKET HGVRAVECRH HSCPMLFATA EAMEAHHKSH YAFHCPHCDF ACSNKHLFRK
361HKKQGHPGSE ELRCTFCPFA TFNPVAYQDH VGKMHAHEKI HQCPECNFAT AHKRVLIRHM
421LLHTGEKPHK CELCDFTCRD VSYLSKHMLT HSNTKDYMCT ECGYVTKWKH YLRVHMRKHA
481GDLRYQCNQC SYRCHRADQL SSHKLRHQGK SLMCEVCAFA CKRKYELQKH MASQHHPGTP
541APLYPCHYCS YQSRHKQAVL SHENCKHTRL REFHCALCDY RTFSNTTLLF HKRKAHGYVP
601GDQAWQLRYA SQEPEGAMQG PTPPPDSEPS NQLSARPEGP GHEPGTVVDP SLDQALPEMS
661EEVNTGRQEG SEAPHGGDLG GSPSPAEVEE GSCTLHLEAL GVELESVTEP PLEEVTETAP
721MEFRPLGLEG PDGLEGPELS SFEGIGTSDL SAEENPLLEK PVSEPSTNPP SLEEAPNNWV
781GTFKTTPPAE TAPLPPLPES ESLLKALRRQ DKEQAEALVL EGRVQMVVIQ GEGRAFRCPH
841CPFITRREKA LNLHSRTGCQ GRREPLLCPE CGASFKQQRG LSTHLLKKCP VLLRKNKGLP
901RPDSPIPLQP VLPGTQASED TESGKPPPAS QEAELLLPKD APLELPREPE ETEEPLATVS
961GSPVPPAGNS LPTEAPKKHC FDPVPPAGNS SPTEAPKKHH LDPVPPAGNS SPTEALKKHR
1021FEQGKFHCNS CPFLCSRLSS ITSHVAEGCR GGRGGGGKRG TPQTQPDVSP LSNGDSAPPK
1081NGSTESSSGD GDTVLVQKQK GARFSCPTCP FSCQQERALR THQIRGCPLE ESGELHCSLC
1141PFTAPAATAL RLHQKRRHPT AAPARGPRPH LQCGDCGFTC KQSRCMQQHR RLKHEGVKPH
1201QCPFCDFSTT RRYRLEAHQS RHTGIGRIPC SSCPQTFGTN SKLRLHRLRV HDKTPTHFCP
1261LCDYSGYLRH DITRHVNSCH QGTPAFACSQ CEAQFSSETA LKQHALRRHP EPAQPAPGSP
1321AETTEGPLHC SRCGLLCPSP ASLRGHTRKQ HPRLECGACQ EAFPSRLALD EHRRQQHFSH
1381RCQLCDFAAR ERVGLVKHYL EQHEETSAAV AASDGDGDAG QPPLHCPFCD FTCRHQLVLD
1441HHVKGHGGTR LYKCTDCAYS TKNRQKITWH SRIHTGEKPY HCHLCPYACA DPSRLKYHMR
1501IHKEERKYLC PECGYKCKWV NQLKYHMTKH TGLKPYQCPE CEYCTNRADA LRVHQETRHR
1561EARAFMCEQC GKAFKTRFLL RTHLRKHSEA KPYVCNVCHR AFRWAAGLRH HALTHTDRHP
1621FFCRLCNYKA KQKFQVVKHV RRHHPDQADP NQGVGKDPTT PTVHLHDVQL EDPSPPAPAA
1681PHTGPEG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1687 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.