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ZNF131 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF131 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF131 antibodies →
$725.00each
Catalog Number: LS-G117345-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF131 (NM_003432), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZBTB35 | Zinc finger protein 131 | PHZ-10 | ZNF131
Origin Species
Human
NCBI GenBank
U09410
SwissProt
P52739

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAEETMECL QEFPEHHKMI LDRLNEQREQ DRFTDITLIV DGHHFKAHKA VLAACSKFFY
61KFFQEFTQEP LVEIEGVSKM AFRHLIEFTY TAKLMIQGEE EANDVWKAAE FLQMLEAIKA
121LEVRNKENSA PLEENTTGKN EAKKRKIAET SNVITESLPS AESEPVEIEV EIAEGTIEVE
181DEGIETLEEV ASAKQSVKYI QSTGSSDDSA LALLADITSK YRQGDRKGQI KEDGCPSDPT
241SKQEHMKSHS TESFKCEICN KRYLRESAWK QHLNCYHLEE GGVSKKQRTG KKIHVCQYCE
301KQFDHFGHFK EHLRKHTGEK PFECPNCHER FARNSTLKCH LTACQTGVGA KKGRKKLYEC
361QVCNSVFNSW DQFKDHLVIH TGDKPNHCTL CDLWFMQGNE LRRHLSDAHN ISERLVTEEV
421LSVETRVQTE PVTSMTIIEQ VGKVHVLPLL QVQVDSAQVT VEQVHPDLLQ DSQVHDSHMS
481ELPEQVQVSY LEVGRIQTEE GTEVHVEELH VERVNQMPVE VQTELLEADL DHVTPEIMNQ
541EERESSQADA AEAAREDHED AEDLETKPTV DSEAEKAENE DRTALPVLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

589 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.