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ZNF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF12 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF12 antibodies →
$725.00each
Catalog Number: LS-G114967-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF12 (NM_006956), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GIOT-3 | HZF11 | KOX3 | Zinc finger protein 12 | Zinc finger protein 325 | ZNF325 | Zinc finger protein 11 | ZNF12 | GIOT3 | Zinc finger protein KOX3
Origin Species
Human
NCBI GenBank
NM_016265
SwissProt
P17014

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNKSLGPVSF KDVAVDFTQE EWQQLDPEQK ITYRDVMLEN YSNLVSVGYH IIKPDVISKL
61EQGEEPWIVE GEFLLQSYPD EVWQTDDLIE RIQEEENKPS RQTVFIETLI EEREYISSDG
121SYARMKADEC SGCGKSLLHI KLEKTHPGDQ AYEFNQNGEP YTLNEESLYQ KIRILEKPFE
181YIECQKAFQK DTVFVNHMEE KPYKWNGSEI AFLQMSDLTV HQTSHMEMKP YECSECGKSF
241CKKSKFIIHQ RTHTGEKPYE CNQCGKSFCQ KGTLTVHQRT HTGEKPYECN ECGKNFYQKL
301HLIQHQRTHS GEKPYECSYC GKSFCQKTHL TQHQRTHSGE RPYVCHDCGK TFSQKSALND
361HQKIHTGVKL YKCSECGKCF CRKSTLTTHL RTHTGEKPYE CNECGKFFSR LSYLTVHYRT
421HSGEKPYECN ECGKTFYLNS ALMRHQRVHT GEKPYECNEC GKLFSQLSYL TIHHRTHSGV
481KPYECSECGK TFYQNSALCR HRRIHKGEKP YECYICGKFF SQMSYLTIHH RIHSGEKPYE
541CSECGKTFCQ NSALNRHQRT HTGEKAYECY ECGKCFSQMS YLTIHHRIHS GEKPFECNEC
601GKAFSRMSYL TVHYRTHSGE KPYECTECGK KFYHKSAFNS HQRIHRRGNM NVIDVGRLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

659 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.