Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF10 / KOX1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101312-100
Catalog Number: LS-G101312
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF10 (NM_015394), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KOX1 | Zinc finger protein 10 | Zinc finger protein 10 (KOX 1) | Zinc finger protein KOX1 | ZNF10
- Origin Species
- Human
- NCBI GenBank
- NM_015394
- SwissProt
- P21506
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDAKSLTAWS RTLVTFKDVF VDFTREEWKL LDTAQQIVYR NVMLENYKNL VSLGYQLTKP61DVILRLEKGE EPWLVEREIH QETHPDSETA FEIKSSVSSR SIFKDKQSCD IKMEGMARND121LWYLSLEEVW KCRDQLDKYQ ENPERHLRQV AFTQKKVLTQ ERVSESGKYG GNCLLPAQLV181LREYFHKRDS HTKSLKHDLV LNGHQDSCAS NSNECGQTFC QNIHLIQFAR THTGDKSYKC241PDNDNSLTHG SSLGISKGIH REKPYECKEC GKFFSWRSNL TRHQLIHTGE KPYECKECGK301SFSRSSHLIG HQKTHTGEEP YECKECGKSF SWFSHLVTHQ RTHTGDKLYT CNQCGKSFVH361SSRLIRHQRT HTGEKPYECP ECGKSFRQST HLILHQRTHV RVRPYECNEC GKSYSQRSHL421VVHHRIHTGL KPFECKDCGK CFSRSSHLYS HQRTHTGEKP YECHDCGKSF SQSSALIVHQ481RIHTGEKPYE CCQCGKAFIR KNDLIKHQRI HVGEETYKCN QCGIIFSQNS PFIVHQIAHT541GEQFLTCNQC GTALVNTSNL IGYQTNHIRE NAYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
573 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.