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ZMYM5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZMYM5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126083-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF237(ZMYM5) (NM_001142684), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYM | Zinc finger | MYM-type 5 | ZNF237 | Zinc finger protein 198-like 1 | HSPC050 | Zinc finger MYM-type protein 5 | Zinc finger protein 237 | ZMYM5 | ZNF198L1
Origin Species
Human
NCBI GenBank
NM_001039649
SwissProt
Q9UJ78

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKCSVGGLE LTEQTPALLG NMAMATSLMD IGDSFGHPAC PLVSRSRNSP VEDDDDDDDV
61VFIESIQPPS ISAPAIADQR NFIFASSKNE KPQGNYSVIP PSSRDLASQK GNISETIVID
121DEEDIETNGG AEKKSSCFIE WGLPGTKNKT NDLDFSTSSL SRSKTKTGVR PFNPGRMNVA
181GDLFQNGEFA THHSPDSWIS QSASFPSNQK QPGVDSLSPV ALLRKQNFQP TAQQQLTKPA
241KITCANCKKP LQKGQTAYQR KGSAHLFCST TCLSSFSHKR TQNTRSIICK KDASTKKANV
301ILPVESSKSF QEFYSTSCLS PCENNWNLKK GVFNKSRCTI CSKLAEIRHE VSVNNVTHKL
361CSNHCFNKYR LANGLIMNCC EHCGEYMPSK STGNNILVIG GQQKRFCCQS CINEYKQMME
421TKSKKLTASE NRKRNAFREE NEKQLYGSSN TLLKKIEGIP EKKEKTSQLQ LSVECGTDTL
481LIQENVNLPP SSTSTIADTF QEQLEEKNFE DSIVPVVLSA DPGTWPRILN IKQRDTLVEN
541VPPQVRNFNF PKDNTGRKFS ETYYTRILPN GEKTTRSWLL YSTSKDSVFC LYCKLFGEGK
601NQLKNENGCK DWQHLSHILS KHEESEMHVN NSVKYSKLKS DLKKNKAIDA AEHRLYENEK
661NDGVLLLYT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

669 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.