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ZMYM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZMYM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109775-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZMYM1 (NM_024772), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYM | Zinc finger MYM-type protein 1 | Zinc finger | MYM-type 1 | ZMYM1 | RP11-181E22.4
Origin Species
Human
NCBI GenBank
NM_024772
SwissProt
Q5SVZ6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKEPLLGGEC DKAVASQLGL LDEIKTEPDN AQEYCHRQQS RTQENELKIN AVFSESASQL
61TAGIQLSLAS SGVNKMLPSV STTAIQVSCA GCKKILQKGQ TAYQRKGSAQ LFCSIPCITE
121YISSASSPVP SKRTCSNCSK DILNPKDVIS VQLEDTTSCK TFCSLSCLSS YEEKRKPFVT
181ICTNSILTKC SMCQKTAIIQ YEVKYQNVKH NLCSNACLSK FHSANNFIMN CCENCGTYCY
241TSSSLSHILQ MEGQSHYFNS SKSITAYKQK PAKPLISVPC KPLKPSDEMI ETTSDLGKTE
301LFCSINCFSA YSKAKMESSS VSVVSVVHDT STELLSPKKD TTPVISNIVS LADTDVALPI
361MNTDVLQDTV SSVTATADVI VDLSKSSPSE PSNAVASSST EQPSVSPSSS VFSQHAIGSS
421TEVQKDNMKS MKISDELCHP KCTSKVQKVK GKSRSIKKSC CADFECLENS KKDVAFCYSC
481QLFCQKYFSC GRESFATHGT SNWKKTLEKF RKHEKSEMHL KSLEFWREYQ FCDGAVSDDL
541SIHSKQIEGN KKYLKLIIEN ILFLGKQCLP LRGNDQSVSS VNKGNFLELL EMRAKDKGEE
601TFRLMNSQVD FYNSTQIQSD IIEIIKTEML QDIVNEINDS SAFSIICDET INSAMKEQLS
661ICVRYPQKSS KAILIKERFL GFVDTEEMTG THLHRTIKTY LQQIGVDMDK IHGQAYDSTT
721NLKIKFNKIA AEFKKEEPRA LYIHCYAHFL DLSIIRFCKE VKELRSALKT LSSLFNTICM
781SGEMLANFRN IYRLSQNKTC KKHISQSCWT VHDRTLLSVI DSLPEIIETL EVIASHSSNT
841SFADELSHLL TLVSKFEFVF CLKFLYRVLS VTGILSKELQ NKTIDIFSLS SKIEAILECF
901II
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

902 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.