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ZMIZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZMIZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101654-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zinc finger MIZ domain containing protein 1(ZMIZ1) (NM_020338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HZIMP10 | MIZ | PIAS-like protein Zimp10 | KIAA1224 | RAI17 | ZMIZ1 | ZIMP10 | Retinoic acid induced 17 | RP11-519K18.1 | TRAFIP10
Origin Species
Human
NCBI GenBank
AF070622
SwissProt
Q9ULJ6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
115.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSMDRHIQQ TNDRLQCIKQ HLQNPANFHN AATELLDWCG DPRAFQRPFE QSLMGCLTVV
61SRVAAQQGFD LDLGYRLLAV CAANRDKFTP KSAALLSSWC EELGRLLLLR HQKSRQSDPP
121GKLPMQPPLS SMSSMKPTLS HSDGSFPYDS VPWQQNTNQP PGSLSVVTTV WGVTNTSQSQ
181VLGNPMANAN NPMNPGGNPM ASGMTTSNPG LNSPQFAGQQ QQFSAKAGPA QPYIQQSMYG
241RPNYPGSGGF GASYPGGPNA PAGMGIPPHT RPPADFTQPA AAAAAAAVAA AAATATATAT
301ATVAALQETQ NKDINQYGPM GPTQAYNSQF MNQPGPRGPA SMGGSMNPAS MAAGMTPSGM
361SGPPMGMNQP RPPGISPFGT HGQRMPQQTY PGPRPQSLPI QNIKRPYPGE PNYGNQQYGP
421NSQFPTQPGQ YPAPNPPRPL TSPNYPGQRM PSQPSSGQYP PPTVNMGQYY KPEQFNGQNN
481TFSGSSYSNY SQGNVNRPPR PVPVANYPHS PVPGNPTPPM TPGSSIPPYL SPSQDVKPPF
541PPDIKPNMSA LPPPPANHND ELRLTFPVRD GVVLEPFRLE HNLAVSNHVF HLRPTVHQTL
601MWRSDLELQF KCYHHEDRQM NTNWPASVQV SVNATPLTIE RGDNKTSHKP LHLKHVCQPG
661RNTIQITVTA CCCSHLFVLQ LVHRPSVRSV LQGLLKKRLL PAEHCITKIK RNFSSVAASS
721GNTTLNGEDG VEQTAIKVSL KCPITFRRIQ LPARGHDCKH VQCFDLESYL QLNCERGTWR
781CPVCNKTALL EGLEVDQYMW GILNAIQHSE FEEVTIDPTC SWRPVPIKSD LHIKDDPDGI
841PSKRFKTMSP SQMIMPNVME MIAALGPGPS PYPLPPPPGG TNSNDYSSQG NNYQGHGNFD
901FPHGNPGGTS MNDFMHGPPQ LSHPPDMPNN MAALEKPLSH PMQETMPHAG SSDQPHPSIQ
961QGLHVPHPSS QSGPPLHHSG APPPPPSQPP RQPPQAAPSS HPHSDLTFNP SSALEGQAGA
1021QGASDMPEPS LDLLPELTNP DELLSYLDPP DLPSNSNDDL LSLFENN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1067 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.