LSBio
ZKSCAN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZKSCAN5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102308-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZKSCAN5 (NM_145102), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1015 | ZFP95 | ZNF914 | Zinc finger protein 95 homolog | ZSCAN37 | Zfp-95 | ZKSCAN5
Origin Species
Human
NCBI GenBank
NM_014569
SwissProt
Q9Y2L8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIMTESREVI DLDPPAETSQ EQEDLFIVKV EEEDCTWMQE YNPPTFETFY QRFRHFQYHE
61ASGPREALSQ LRVLCCEWLR PELHTKEQIL ELLVLEQFLT ILPEEFQPWV REHHPESGEE
121AVAVIENIQR ELEERRQQIV ACPDVLPRKM ATPGAVQESC SPHPLTVDTQ PEQAPQKPRL
181LEENALPVLQ VPSLPLKDSQ ELTASLLSTG SQKLVKIEEV ADVAVSFILE EWGHLDQSQK
241SLYRDDRKEN YGSITSMGYE SRDNMELIVK QISDDSESHW VAPEHTERSV PQDPDFAEVS
301DLKGMVQRWQ VNPTVGKSRQ NPSQKRDLDA ITDISPKQST HGERGHRCSD CGKFFLQASN
361FIQHRRIHTG EKPFKCGECG KSYNQRVHLT QHQRVHTGEK PYKCQVCGKA FRVSSHLVQH
421HSVHSGERPY GCNECGKNFG RHSHLIEHLK RHFREKSQRC SDKRSKNTKL SVKKKISEYS
481EADMELSGKT QRNVSQVQDF GEGCEFQGKL DRKQGIPMKE ILGQPSSKRM NYSEVPYVHK
541KSSTGERPHK CNECGKSFIQ SAHLIQHQRI HTGEKPFRCE ECGKSYNQRV HLTQHQRVHT
601GEKPYTCPLC GKAFRVRSHL VQHQSVHSGE RPFKCNECGK GFGRRSHLAG HLRLHSREKS
661HQCRECGEIF FQYVSLIEHQ VLHMGQKNEK NGICEEAYSW NLTVIEDKKI ELQEQPYQCD
721ICGKAFGYSS DLIQHYRTHT AEKPYQCDIC RENVGQCSHT KQHQKIYSST KSHQCHECGR
781GFTLKSHLNQ HQRIHTGEKP FQCKECGMNF SWSCSLFKHL RSHERTDPIN TLSVEGSLL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.