Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZKSCAN1 / ZNF36 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117352-100
Catalog Number: LS-G117352
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZKSCAN1 (NM_003439), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZNF139 | ZNF36 | ZSCAN33 | ZKSCAN1 | Zinc finger protein 36 | KOX18 | PHZ-37 | Zinc finger protein 139 | Zinc finger protein KOX18
- Origin Species
- Human
- NCBI GenBank
- U09848
- SwissProt
- P17029
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMTAESREAT GLSPQAAQEK DGIVIAKVEE EDEEDHMWGQ DSTLQDTPPP DPEIFRQRFR61RFCYQNTFGP REALSRLKEL CHQWLRPEIN TKEQILELLV LEQFLSILPK ELQVWLQEYR121PDSGEEAVTL LEDLELDLSG QQVPGQVHGP EMLARGMVPL DPVQESSSFD LHHEATQSHF181KHSSRKPRLL QSRALPAAHI PAPPHEGSPR DQAMASALFT ADSQAMVKIE DMAVSLILEE241WGCQNLARRN LSRDNRQENY GSAFPQGGEN RNENEESTSK AETSEDSASR GETTGRSQKE301FGEKRDQEGK TGERQQKNPE EKTRKEKRDS GPAIGKDKKT ITGERGPREK GKGLGRSFSL361SSNFTTPEEV PTGTKSHRCD ECGKCFTRSS SLIRHKIIHT GEKPYECSEC GKAFSLNSNL421VLHQRIHTGE KPHECNECGK AFSHSSNLIL HQRIHSGEKP YECNECGKAF SQSSDLTKHQ481RIHTGEKPYE CSECGKAFNR NSYLILHRRI HTREKPYKCT KCGKAFTRSS TLTLHHRIHA541RERASEYSPA SLDAFGAFLK SCVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
563 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.