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ZIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZIM2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101311-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZIM2 (NM_015363), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 656 | Zinc finger | imprinted 2 | Zinc finger imprinted 2 | ZIM2 | ZNF656
Origin Species
Human
NCBI GenBank
NM_015363
SwissProt
Q9NZV7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYQPEDDNNS DVTSDDDMTR NRRESSPPHS VHSFSGDRDW DRRGRSRDME PRDRWSHTRN
61PRSRMPPRDL SLPVVAKTSF EMDREDDRDS RAYESRSQDA ESYQNVVDLA EDRKPHNTIQ
121DNMENYRKLL SLGFLAQDSV PAEKRNTEML DNLPSAGSQF PDFKHLGTFL VFEELVTFED
181VLVDFSPEEL SSLSAAQRNL YREVMLENYR NLVSLGHQFS KPDIISRLEE EESYAMETDS
241RHTVICQGES HDDPLEPHQG NQEKLLTPIT MNDPKTLTPE RSYGSDEFER SSNLSKQSKD
301PLGKDPQEGT APGICTSPQS ASQENKHNRC EFCKRTFSTQ VALRRHERIH TGKKPYECKQ
361CAEAFYLMPH LNRHQKTHSG RKTSGCNEGR KPSVQCANLC ERVRIHSQED YFECFQCGKA
421FLQNVHLLQH LKAHEAARVL PPGLSHSKTY LIRYQRKHDY VGERACQCCD CGRVFSRNSY
481LIQHYRTHTQ ERPYQCQLCG KCFGRPSYLT QHYQLHSQEK TVECDHC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

527 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.