LSBio
ZHX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZHX3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113505-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZHX3 (NM_015035), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0395 | ZHX3 | TIX1 | Triple homeobox 1 | Triple homeobox protein 1 | Zinc fingers and homeoboxes 3
Origin Species
Human
NCBI GenBank
AB007855
SwissProt
Q9H4I2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MASKRKSTTP CMIPVKTVVL QDASMEAQPA ETLPEGPQQD LPPEASAASS EAAQNPSSTD
61GSTLANGHRS TLDGYLYSCK YCDFRSHDMT QFVGHMNSEH TDFNKDPTFV CSGCSFLAKT
121PEGLSLHNAT CHSGEASFVW NVAKPDNHVV VEQSIPESTS TPDLAGEPSA EGADGQAEII
181ITKTPIMKIM KGKAEAKKIH TLKENVPSQP VGEALPKLST GEMEVREGDH SFINGAVPVS
241QASASSAKNP HAANGPLIGT VPVLPAGIAQ FLSLQQQPPV HAQHHVHQPL PTAKALPKVM
301IPLSSIPTYN AAMDSNSFLK NSFHKFPYPT KAELCYLTVV TKYPEEQLKI WFTAQRLKQG
361ISWSPEEIED ARKKMFNTVI QSVPQPTITV LNTPLVASAG NVQHLIQAAL PGHVVGQPEG
421TGGGLLVTQP LMANGLQATS SPLPLTVTSV PKQPGVAPIN TVCSNTTSAV KVVNAAQSLL
481TACPSITSQA FLDASIYKNK KSHEQLSALK GSFCRNQFPG QSEVEHLTKV TGLSTREVRK
541WFSDRRYHCR NLKGSRAMIP GDHSSIIIDS VPEVSFSPSS KVPEVTCIPT TATLATHPSA
601KRQSWHQTPD FTPTKYKERA PEQLRALESS FAQNPLPLDE ELDRLRSETK MTRREIDSWF
661SERRKKVNAE ETKKAEENAS QEEEEAAEDE GGEEDLASEL RVSGENGSLE MPSSHILAER
721KVSPIKINLK NLRVTEANGR NEIPGLGACD PEDDESNKLA EQLPGKVSCK KTAQQRHLLR
781QLFVQTQWPS NQDYDSIMAQ TGLPRPEVVR WFGDSRYALK NGQLKWYEDY KRGNFPPGLL
841VIAPGNRELL QDYYMTHKML YEEDLQNLCD KTQMSSQQVK QWFAEKMGEE TRAVADTGSE
901DQGPGTGELT AVHKGMGDTY SEVSENSESW EPRVPEASSE PFDTSSPQAG RQLETD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

956 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.