Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZHX2 / RAF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113574-100
Catalog Number: LS-G113574
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZHX2 (NM_014943), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AFR1 | Alpha-fetoprotein regulator 1 | AFP regulator 1 | KIAA0854 | Regulator of AFP | Transcription factor ZHX2 | Zinc fingers and homeoboxes 2 | Zinc-fingers and homeoboxes 2 | RAF | ZHX2
- Origin Species
- Human
- NCBI GenBank
- NM_014943
- SwissProt
- Q9Y6X8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKRKSTTP CMVRTSQVVE QDVPEEVDRA KEKGIGTPQP DVAKDSWAAE LENSSKENEV61IEVKSMGESQ SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK121KYDSLSDHNS KFHPGEANFK LKLIKRNNQT VLEQSIETTN HVVSITTSGP GTGDSDSGIS181VSKTPIMKPG KPKADAKKVP KKPEEITPEN HVEGTARLVT DTAEILSRLG GVELLQDTLG241HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK301HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLAPTKVTQP361ILQTALPCQI LGQTSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR421PHIAQVPEPP PKVANPPLTP ASDRKKTKEQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR481SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ LAIAASRHGR TYHAYPDFAP QKFKEKTQGQ541VKILEDSFLK SSFPTQAELD RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK601GQDVGAPNGA LSRLDQLSGA QLTSSLPSPS PAIAKSQEQV HLLRSTFART QWPTPQEYDQ661LAAKTGLVRT EIVRWFKENR CLLKTGTVKW MEQYQHQPMA DDHGYDAVAR KATKPMAESP721KNGGDVVPQY YKDPKKLCEE DLEKLVTRVK VGSEPAKDCL PAKPSEATSD RSEGSSRDGQ781GSDENEESSV VDYVEVTVGE EDAISDRSDS WSQAAAEGVS ELAESDSDCV PAEAGQATRTRPLEQKLISEEDLAANDILDYKDDDDKV
837 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.