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ZGPAT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZGPAT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108752-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZGPAT (NM_032527), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ583P15.3 | GPATC6 | KIAA1847 | RP4-583P15.3 | ZGPAT | ZC3HDC9 | ZIP | ZC3H9 | GPATCH6
Origin Species
Human
NCBI GenBank
NM_032527
SwissProt
Q8N5A5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDEESLESAL QTYRAQLQQV ELALGAGLDS SEQADLRQLQ GDLKELIELT EASLVSVRKS
61SLLAALDEER PGRQEDAEYQ AFREAITEAV EAPAAARGSG SETVPKAEAG PESAAGGQEE
121EEGEDEEELS GTKVSAPYYS SWGTLEYHNA MVVGTEEAED GSAGVRVLYL YPTHKSLKPC
181PFFLEGKCRF KENCRFSHGQ VVSLDELRPF QDPDLSSLQA GSACLAKHQD GLWHAARITD
241VDNGYYTVKF DSLLLREAVV EGDGILPPLR TEATESDSDS DGTGDSSYAR VVGSDAVDSA
301QSSALCPSLA VVGSDAVDSG TCSSAFAGWE VHTRGIGSRL LTKMGYEFGK GLGRHAEGRV
361EPIHAVVLPR GKSLDQCVET LQKQTRVGKA GTNKPPRCRG RGARPGGRPA PRNVFDFLNE
421KLQGQAPGAL EAGAAPAGRR SKDMYHASKS AKRALSLRLF QTEEKIERTQ RDIRSIQEAL
481ARNAGRHSVA SAQLQEKLAG AQRQLGQLRA QEAGLQQEQR KADTHKKMTE F
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

531 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.