ZFYVE9 / SARA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114715-100
Catalog Number: LS-G114715
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SARA(ZFYVE9) (NM_007324), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSARA | Madh-interacting protein | Receptor activation anchor | SMADIP | MADHIP | ZFYVE9 | Novel serine protease | NSP | SARA
- Origin Species
- Human
- NCBI GenBank
- NM_004799
- SwissProt
- O95405
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 150.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MENYFQAEAY NLDKVLDEFE QNEDETVSST LLDTKWNKIL DPPSHRLSFN PTLASVNESA61VSNESQPQLK VFSLAHSAPL TTEEEDHCAN GQDCNLNPEI ATMWIDENAV AEDQLIKRNY121SWDDQCSAVE VGEKKCGNLA CLPDEKNVLV VAVMHNCDKR TLQNDLQDCN NYNSQSLMDA181FSCSLDNENR QTDQFSFSIN ESTEKDMNSE KQMDPLNRPK TEGRSVNHLC PTSSDSLASV241CSPSQLKDDG SIGRDPSMSA ITSLTVDSVI SSQGTDGCPA VKKQENYIPD EDLTGKISSP301RTDLGSPNSF SHMSEGILMK KEPAEESTTE ESLRSGLPLL LKPDMPNGSG RNNDCERCSD361CLVPNEVRAD ENEGYEHEET LGTTEFLNMT EHFSESQDMT NWKLTKLNEM NDSQVNEEKE421KFLQISQPED TNGDSGGQCV GLADAGLDLK GTCISESEEC DFSTVIDTPA ANYLSNGCDS481YGMQDPGVSF VPKTLPSKED SVTEEKEIEE SKSECYSNIY EQRGNEATEG SGLLLNSTGD541LMKKNYLHNF CSQVPSVLGQ SSPKVVASLP SISVPFGGAR PKQPSNLKLQ IPKPLSDHLQ601NDFPANSGNN TKNKNDILGK AKLGENSATN VCSPSLGNIS NVDTNGEHLE SYEAEISTRP661CLALAPDSPD NDLRAGQFGI SARKPFTTLG EVAPVWVPDS QAPNCMKCEA RFTFTKRRHH721CRACGKVFCA SCCSLKCKLL YMDRKEARVC VICHSVLMNV AQPREQRRVW FADGILPNGE781VADAAKLTMN GTSSAGTLAV SHDPVKPVTT SPLPAETDIC LFSGSITQVG SPVGSAMNLI841PEDGLPPILI STGVKGDYAV EEKPSQISVM QQLEDGGPDP LVFVLNANLL SMVKIVNYVN901RKCWCFTTKG MHAVGQSEIV ILLQCLPDEK CLPKDIFNHF VQLYRDALAG NVVSNLGHSF961FSQSFLGSKE HGGFLYVTST YQSLQDLVLP TPPYLFGILI QKWETPWAKV FPIRLMLRLG1021AEYRLYPCPL FSVRFRKPLF GETGHTIMNL LADFRNYQYT LPVVQGLVVD MEVRKTSIKI1081PSNRYNEMMK AMNKSNEHVL AGGACFNEKA DSHLVCVQND DGNYQTQAIS IHNQPRKVTG1141ASFFVFSGAL KSSSGYLAKS SIVEDGVMVQ ITAENMDSLR QALREMKDFT ITCGKADAEE1201PQEHIHIQWV DDDKNVSKGV VSPIDGKSME TITNVKIFHG SEYKANGKVI RWTEVFFLEN1261DDQHNCLSDP ADHSRLTEHV AKAFCLALCP HLKLLKEDGM TKLGLRVTLD SDQVGYQAGS1321NGQPLPSQYM NDLDSALVPV IHGGACQLSE GPVVMELIFY ILENIVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1366 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.