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ZFY Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFY Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126609-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFY (NM_001145276), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZFY | Zinc finger protein | Y-linked | ZNF911
Origin Species
Human
NCBI GenBank
NM_003411
SwissProt
P08048

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDEDEFELQP QEPNSFFDGI VDDAGKIEHD GSTGVTIDAE SEMDPCKVDS TCPEVIKVYI
61FKADPGEDDL GGTVDIVESE PENDHGVELL DQNSSIRVPR EKMVYMTVND SQQEDEDLNV
121AEIADEVYME VIVGEEDAAV AAAAAAVHEQ QIDEDEMKTF VPIAWAAAYG NNSDGIENRN
181GTASALLHID ESAGLGRLAK QKPKKKRRPD SRQYQTAIII GPDGHPLTVY PCMICGKKFK
241SRGFLKRHMK NHPEHLAKKK YHCTDCDYTT NKKISLHNHL ESHKLTSKAE KAIECDECGK
301HFSHAGALFT HKMVHKEKGA NKMHKCKFCE YETAEQGLLN RHLLAVHSKN FPHICVECGK
361GFRHPSELRK HMRIHTGEKP YQCQYCEYRS ADSSNLKTHI KTKHSKEMPF KCDICLLTFS
421DTKEVQQHTL VHQESKTHQC LHCDHKSSNS SDLKRHVISV HTKDYPHKCE MCEKGFHRPS
481ELKKHVAVHK GKKMHQCRHC DFKIADPFVL SRHILSVHTK DLPFRCKRCR KGFRQQNELK
541KHMKTHSGRK VYQCEYCEYS TTDASGFKRH VISIHTKDYP HRCEYCKKGF RRPSEKNQHI
601MRHHKEVGLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.