LSBio
ZFX Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFX Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZFX antibodies →
$725.00each
Catalog Number: LS-G117358-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFX (NM_003410), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
X-linked zinc finger protein | ZFX | Zinc finger protein | X-linked | ZNF926
Origin Species
Human
NCBI GenBank
NM_003410
SwissProt
P17010

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDEDGLELQQ EPNSFFDATG ADGTHMDGDQ IVVEVQETVF VSDVVDSDIT VHNFVPDDPD
61SVVIQDVIED VVIEDVQCPD IMEEADVSET VIIPEQVLDS DVTEEVSLAH CTVPDDVLAS
121DITSASMSMP EHVLTGDSIH VSDVGHVGHV GHVEHVVHDS VVEAEIVTDP LTTDVVSEEV
181LVADCASEAV IDANGIPVDQ QDDDKGNCED YLMISLDDAG KIEHDGSSGM TMDTESEIDP
241CKVDGTCPEV IKVYIFKADP GEDDLGGTVD IVESEPENDH GVELLDQNSS IRVPREKMVY
301MTVNDSQPED EDLNVAEIAD EVYMEVIVGE EDAAAAAAAA AVHEQQMDDN EIKTFMPIAW
361AAAYGNNSDG IENRNGTASA LLHIDESAGL GRLAKQKPKK RRRPDSRQYQ TAIIIGPDGH
421PLTVYPCMIC GKKFKSRGFL KRHMKNHPEH LAKKKYRCTD CDYTTNKKIS LHNHLESHKL
481TSKAEKAIEC DECGKHFSHA GALFTHKMVH KEKGANKMHK CKFCEYETAE QGLLNRHLLA
541VHSKNFPHIC VECGKGFRHP SELKKHMRIH TGEKPYQCQY CEYRSADSSN LKTHVKTKHS
601KEMPFKCDIC LLTFSDTKEV QQHALIHQES KTHQCLHCDH KSSNSSDLKR HIISVHTKDY
661PHKCDMCDKG FHRPSELKKH VAAHKGKKMH QCRHCDFKIA DPFVLSRHIL SVHTKDLPFR
721CKRCRKGFRQ QSELKKHMKT HSGRKVYQCE YCEYSTTDAS GFKRHVISIH TKDYPHRCEY
781CKKGFRRPSE KNQHIMRHHK EVGLP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

805 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.