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ZFR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFR2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113435-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFR2 (NM_015174), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZFR2 | KIAA1086
Origin Species
Human
NCBI GenBank
NM_015174

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATSQYFDFA QGGGPQYSAQ PPTLPLPTVG ASYTAQPTPG MDPAVNPAFP PAAPAGYGGY
61QPHSGQDFAY GSRPQEPVPT ATTMATYQDS YSYGQSAAAR SYEDRPYFQS AALQSGRMTA
121ADSGQPGTQE ACGQPSPHGS HSHAQPPQQA PIVESGQPAS TLSSGYTYPT ATGVQPESSA
181SIVTSYPPPS YNPTCTAYTA PSYPNYDASV YSAASPFYPP AQPPPPPGPP QQLPPPPAPA
241GSGSSPRADS KPPLPSKLPR PKAGPRQLQL HYCDICKISC AGPQTYREHL GGQKHRKKEA
301AQKTGVQPNG SPRGVQAQLH CDLCAVSCTG ADAYAAHIRG SKHQKVFKLH AKLGKPIPTL
361EPALATESPP GAEAKPTSPT GPSVCASSRP ALAKRPVASK ALCEGPPEPQ AAGCRPQWGK
421PAQPKLEGPG APTQGGSKEA PAGCSDAQPV GPEYVEEVFS DEGRVLRFHC KLCECSFNDL
481NAKDLHVRGR RHRLQYRKKV NPDLPIATEP SSRARKVLEE RMRKQRHLAE ERLEQLRRWH
541AERRRLEEEP PQDVPPHAPP DWAQPLLMGR PESPASAPLQ PGRRPASSDD RHVMCKHATI
601YPTEQELLAV QRAVSHAERA LKLVSDTLAE EDRGRREEEG DKRSSVAPQT RVLKGVMRVG
661ILAKGLLLRG DRNVRLALLC SEKPTHSLLR RIAQQLPRQL QMVTEDEYEV SSDPEANIVI
721SSCEEPRMQV TISVTSPLMR EDPSTDPGVE EPQADAGDVL SPKKCLESLA ALRHARWFQA
781RASGLQPCVI VIRVLRDLCR RVPTWGALPA WAMELLVEKA VSSAAGPLGP GDAVRRVLEC
841VATGTLLTDG PGLQDPCERD QTDALEPMTL QEREDVTASA QHALRMLAFR QTHKVLGMDL
901LPPRHRLGAR FRKRQRGPGE GEEGAGEKKR GRRGGEGLVT RP
LEQKLISEEDLAANDILDYKDDDDKV

942 aa + 26 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.