Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZFP82 / ZNF545 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107510-100
Catalog Number: LS-G107510
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF545(ZFP82) (NM_133466), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1948 | ZFP82 | ZNF545 | Zfp-82 | Zinc finger protein 82 homolog | Zinc finger protein 545 | ZFP82 zinc finger protein | Zinc finger protein 85
- Origin Species
- Human
- NCBI GenBank
- NM_133466
- SwissProt
- Q8N141
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALRSVMFSD VSIDFSPEEW EYLDLEQKDL YRDVMLENYS NLVSLGCFIS KPDVISSLEQ61GKEPWKVVRK GRRQYPDLET KYETKKLSLE NDIYEINLSQ WKIMERIENH GLKGLILKND121WESTGKIEGQ ERPQEGYFSS VKMPSEKVSS YQKRTSVTPH QRLHFVDKPY ECKECGKAFR181VRQQLTFHHR IHTGEKPYEC KECGMAFRQT AHLTRHQRLH SGEKLYECKE CGEAFICGAD241LRVHQKMHIG EKPYECKECG KAFRVRGQLT LHQRIHTGEK PYVCKECGKA FRQYAHLTRH301QKLNSADRLY ECKECGKAFL CGSGLRVHHK LHTGEKPYEC KECGKAFRVR QQLTLHQRIH361TGEKPYECKE CGKTFSRGYH LILHHRIHTG EKPYECKECW KAFSRYSQLI SHQSIHIGVK421PYDCKECGKA FRLLSQLTQH QSIHIGEKPY KCKECGKAFR LRQKLTLHQS IHTGEKPFEC481KECRKAFRLN SSLIQHLRIH SGEKPYECKE CKKAFRQHSH LTHHLKIHNV KITRTRPLEQKLISEEDLAANDILDYKDDDDKV
532 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.