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ZFP64 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFP64 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103330-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP64 (NM_199427), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ548G19.1 | Zfp-64 | ZFP64 zinc finger protein | Zinc finger protein 338 | ZNF338 | ZFP64 | Zinc finger protein 64 | DJ831D17.1
Origin Species
Human
NCBI GenBank
NM_018197
SwissProt
Q9NPA5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNASSEGESF AGSVQIPGGT TVLVELTPDI HICGICKQQF NNLDAFVAHK QSGCQLTGTS
61AAAPSTVQFV SEETVPATQT QTTTRTITSE TQTITVSAPE FVFEHGYQTY LPTESNENQT
121ATVISLPAKS RTKKPTTPPA QKRLNCCYPG CQFKTAYGMK DMERHLKIHT GDKPHKCEVC
181GKCFSRKDKL KTHMRCHTGV KPYKCKTCDY AAADSSSLNK HLRIHSDERP FKCQICPYAS
241RNSSQLTVHL RSHTASELDD DVPKANCLST ESTDTPKAPV ITLPSEAREQ MATLGERTFN
301CCYPGCHFKT VHGMKDLDRH LRIHTGDKPH KCEFCDKCFS RKDNLTMHMR CHTSVKPHKC
361HLCDYAAVDS SSLKKHLRIH SDERPYKCQL CPYASRNSSQ LTVHLRSHTG DTPFQCWLCS
421AKFKISSDLK RHMIVHSGEK PFKCEFCDVR CTMKANLKSH IRIKHTFKCL HCAFQGRDRA
481DLLEHSRLHQ ADHPEKCPEC SYSCSSAAAL RVHSRVHCKD CPFKCDFCSF DTKRPSSLAK
541HVDKVHRDEA KTENRAPLGK EGLREGSSQH VAKIVTQRAF RCETCGASFV RDDSLRCHKK
601QHSDQSENKN SDLVTFPPES GASGQLSTLV SVGQLEAPLE PSQDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

645 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.