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ZFP37 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFP37 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP37 (NM_003408), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZFP37 | Zinc finger protein 37 homolog | ZNF906 | Zfp-37 | ZFP37 zinc finger protein
Origin Species
Human
NCBI GenBank
NM_003408
SwissProt
Q9Y6Q3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVSSGVQIL TKPETVDRRR SAETTKEAGR PLEMAVSEPE ASAAEWKQLD PAQSNLYNDV
61MLENYCNQAS MGCQAPKPDM ISKLEKGEAP WLGKGKRPSQ GCPSKIARPK QKETDGKVQK
121DDDQLENIQK SQNKLLREVA VKKKTQAKKN GSDCGSLGKK NNLHKKHVPS KKRLLKFESC
181GKILKQNLDL PDHSRNCVKR KSDAAKEHKK SFNHSLSDTR KGKKQTGKKH EKLSSHSSSD
241KCNKTGKKHD KLCCHSSSHI KQDKIQTGEK HEKSPSLSSS TKHEKPQACV KPYECNQCGK
301VLSHKQGLID HQRVHTGEKP YECNECGIAF SQKSHLVVHQ RTHTGEKPYE CIQCGKAHGH
361KHALTDHLRI HTGEKPYECA ECGKTFRHSS NLIQHVRSHT GEKPYECKEC GKSFRYNSSL
421TEHVRTHTGE IPYECNECGK AFKYSSSLTK HMRIHTGEKP FECNECGKAF SKKSHLIIHQ
481RTHTKEKPYK CNECGKAFGH SSSLTYHMRT HTGESPFECN QCGKGFKQIE GLTQHQRVHT
541GEKPYECNEC GKAFSQKSHL IVHQRTHTGE KPYECNECEK AFNAKSQLVI HQRSHTGEKP
601YECNECGKTF KQNASLTKHV KTHSEDKSHE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

630 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.