LSBio
ZFP30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFP30 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZFP30 antibodies →
$504.00each
Catalog Number: LS-G113616-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP30 (NM_014898), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zfp-30 | Zinc finger protein 745 | ZNF745 | ZFP30 | ZFP30 zinc finger protein | KIAA0961 | Zinc finger protein 30 homolog
Origin Species
Human
NCBI GenBank
AB023178
SwissProt
Q9Y2G7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARDLVMFRD VAVDFSQEEW ECLNSYQRNL YRDVILENYS NLVSLAGCSI SKPDVITLLE
61QGKEPWMVVR DEKRRWTLDL ESRYDTKKLF QGKDIYEMNL SQWKVMERIK SCGLEEQESP
121HEVCFRQVTK TTSEKMPTYR KLTSLPLYQK SHNREKPYEC GECGKAFRVR QQLTFHQRIH
181TGEKPYECKE CGKAFRQCAH LSRHQRIHTS DKLYECKKCG KIFTCGSDLR VHQRIHIGEK
241PYECKECGKA FRVRGQLNLH QRIHTGEKPY ECKECGKAFR QYAHLTRHQR LNIAEKCYEC
301KECGQAFLCS TGLRLHHKLH TGEKPYECKE CGKAFRVRQQ LTLHQRIHTG EKPYDCKECG
361KTFSRGYHLT LHQRIHTGEK PYECKECQKF FRRYSELISH QGIHIGEKPY ECKECGKAFR
421LFSQLTQHQS IHFGEKPFKC KECEKTFRLL SQLTQHQSIH TGEKPYDCKE CGKAFRLHSS
481LIQHQRIHSG EKPYKCKECK KAFRQHSHLT YHQRIHNVT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

519 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.