LSBio
ZFP28 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFP28 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZFP28 antibodies →
$725.00each
Catalog Number: LS-G110983-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFP28 (NM_020828), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1431 | Mkr5 | Zinc finger factor X6 | ZFP28 zinc finger protein | ZFP28 | Zinc finger protein 28 homolog | Zfp-28
Origin Species
Human
NCBI GenBank
AB037852
SwissProt
Q8NHY6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGAASASVR EPTPLPGRGA PRTKPRAGRG PTVGTPATLV LPGRGRPRSR NGLASKGQRG
61AAPTGPGHRA LPSRDTALPQ ERNKKLEAVG TGIEPKAMSQ GLVTFGDVAV DFSQEEWEWL
121NPIQRNLYRK VMLENYRNLA SLGLCVSKPD VISSLEQGKE PWTVKRKMTR AWCPDLKAVW
181KIKELPLKKD FCEGKLSQAV ITERLTSYNL EYSLLGEHWD YDALFETQPG LVTIKNLAVD
241FRQQLHPAQK NFCKNGIWEN NSDLGSAGHC VAKPDLVSLL EQEKEPWMVK RELTGSLFSG
301QRSVHETQEL FPKQDSYAEG VTDRTSNTKL DCSSFRENWD SDYVFGRKLA VGQETQFRQE
361PITHNKTLSK ERERTYNKSG RWFYLDDSEE KVHNRDSIKN FQKSSVVIKQ TGIYAGKKLF
421KCNECKKTFT QSSSLTVHQR IHTGEKPYKC NECGKAFSDG SSFARHQRCH TGKKPYECIE
481CGKAFIQNTS LIRHWRYYHT GEKPFDCIDC GKAFSDHIGL NQHRRIHTGE KPYKCDVCHK
541SFRYGSSLTV HQRIHTGEKP YECDVCRKAF SHHASLTQHQ RVHSGEKPFK CKECGKAFRQ
601NIHLASHLRI HTGEKPFECA ECGKSFSISS QLATHQRIHT GEKPYECKVC SKAFTQKAHL
661AQHQKTHTGE KPYECKECGK AFSQTTHLIQ HQRVHTGEKP YKCMECGKAF GDNSSCTQHQ
721RLHTGQRPYE CIECGKAFKT KSSLICHRRS HTGEKPYECS VCGKAFSHRQ SLSVHQRIHS
781GKKPYECKEC RKTFIQIGHL NQHKRVHTGE RSYNYKKSRK VFRQTAHLAH HQRIHTGESS
841TCPSLPSTSN PVDLFPKFLW NPSSLPSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

868 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.