Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZFP219 / ZNF219 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G118970-100
Catalog Number: LS-G118970
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zfp219(ZNF219) (NM_001101672), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZFP219 | ZNF219 | Zinc finger protein 219
- Origin Species
- Human
- NCBI GenBank
- NM_016423
- SwissProt
- Q9P2Y4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGSRPRAPS GHLAPSPPAF DGELDLQRYS NGPAVSAGSL GMGAVSWSES RAGERRFPCP61VCGKRFRFNS ILALHLRAHP GAQAFQCPHC GHRAAQRALL RSHLRTHQPE RPRSPAARLL121LELEERALLR EARLGRARSS GGMQATPATE GLARPQAPSS SAFRCPYCKG KFRTSAERER181HLHILHRPWK CGLCSFGSSQ EEELLHHSLT AHGAPERPLA ATSAAPPPQP QPPPQPEPRS241VPQPEPEPEP EREATPTPAP AAPEEPPAPP EFRCQVCGQS FTQSWFLKGH MRKHKASFDH301ACPVCGRCFK EPWFLKNHMK VHASKLGPLR APGPASGPAR APQPPDLGLL AYEPLGPALL361LAPAPTPAER REPPSLLGYL SLRAGEGRPN GEGAEPGPGR SFGGFRPLSS ALPARARRHR421AEEPEEEEEV VEAEEETWAR GRSLGSLASL HPRPGEGPGH SASAAGAQAR STATQEENGL481LVGGTRPEGG RGATGKDCPF CGKSFRSAHH LKVHLRVHTG ERPYKCPHCD YAGTQSGSLK541YHLQRHHREQ RSGAGPGPPP EPPPPSQRGS APQSGAKPSP QPATWVEGAS SPRPPSSGAG601PGSRRKPASP GRTLRNGRGG EAEPLDLSLR AGPGGEAGPG GALHRCLFCP FATGAPELMA661LHLQVHHSRR ARGRRPPQAD ASPPYARVPS GETPPSPSQE GEEGSGLSRP GEAGLGGQERSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
720 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.