Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Zfp105 / ZNF35 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G117368-100
Catalog Number: LS-G117368
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF35 (NM_003420), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HF.10 | Zinc finger protein HF.10 | Zfp105 | Zinc finger protein 35 | ZNF35 | HF10
- Origin Species
- Human
- NCBI GenBank
- NM_003420
- SwissProt
- P13682
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTAELREAMA LAPWGPVKVK KEEEEEENFP GQASSQQVHS ENIKVWAPVQ GLQTGLDGSE61EEEKGQNISW DMAVVLKATQ EAPAASTLGS YSLPGTLAKS EILETHGTMN FLGAETKNLQ121LLVPKTEICE EAEKPLIISE RIQKADPQGP ELGEACEKGN MLKRQRIKRE KKDFRQVIVN181DCHLPESFKE EENQKCKKSG GKYSLNSGAV KNPKTQLGQK PFTCSVCGKG FSQSANLVVH241QRIHTGEKPF ECHECGKAFI QSANLVVHQR IHTGQKPYVC SKCGKAFTQS SNLTVHQKIH301SLEKTFKCNE CEKAFSYSSQ LARHQKVHIT EKCYECNECG KTFTRSSNLI VHQRIHTGEK361PFACNDCGKA FTQSANLIVH QRSHTGEKPY ECKECGKAFS CFSHLIVHQR IHTAEKPYDC421SECGKAFSQL SCLIVHQRIH SGDLPYVCNE CGKAFTCSSY LLIHQRIHNG EKPYTCNECG481KAFRQRSSLT VHQRTHTGEK PYECEKCGAA FISNSHLMRH HRTHLVETRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.