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ZFAT Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZFAT Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZFAT antibodies →
$725.00each
Catalog Number: LS-G130387-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZFAT (NM_001174158), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AITD3 | KIAA1485 | ZFAT1 | Zinc finger protein 406 | ZNF406 | ZFAT | Zinc finger protein ZFAT
Origin Species
Human
NCBI GenBank
NM_020863
SwissProt
Q9P243

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCKCCNLFSP NQSELLSHVS EKHMEEGVNV DEIIIPLRPL STPEPPNSSK TGDEFLVMKR
61KRGRPKGSTK KSSTEEELAE NIVSPTEDSP LAPEEGNSLP PSSLECSKCC RKFSNTRQLR
121KHICIIVLNL GEEEGEAGNE SDLELEKKCK EDDREKASKR PRSQKTEKVQ KISGKEARQL
181SGAKKPIISV VLTAHEAIPG ATKIVPVEAG PPETGATNSE TTSADLVPRR GYQEYAIQQT
241PYEQPMKSSR LGPTQLKIFT CEYCNKVFKF KHSLQAHLRI HTNEKPYKCP QCSYASAIKA
301NLNVHLRKHT GEKFACDYCS FTCLSKGHLK VHIERVHKKI KQHCRFCKKK YSDVKNLIKH
361IRDAHDPQDK KVKEALDELC LMTREGKRQL LYDCHICERK FKNELDRDRH MLVHGDKWPF
421ACELCGHGAT KYQALELHVR KHPFVYVCAV CRKKFVSSIR LRTHIKEVHG AAQEALVFTS
481SINQSFCLLE PGGDIQQEAL GDQLQLVEEE FALQGVNALK EEACPGDTQL EEGRKEPEAP
541GEMPAPAVHL ASPQAESTAL PPCELETTVV SSSDLHSQEV VSDDFLLKND TSSAEAHAAP
601EKPPDMQHRS SVQTQGEVIT LLLSKAQSAG SDQESHGAQS PLGEGQNMAV LSAGDPDPSR
661CLRSNPAEAS DLLPPVAGGG DTITHQPDSC KAAPEHRSGI TAFMKVLNSL QKKQMNTSLC
721ERIRKVYGDL ECEYCGKLFW YQVHFDMHVR THTREHLYYC SQCHYSSITK NCLKRHVIQK
781HSNILLKCPT DGCDYSTPDK YKLQAHLKVH TALDKRSYSC PVCEKSFSED RLIKSHIKTN
841HPEVSMSTIS EVLGRRVQLK GLIGKRAMKC PYCDFYFMKN GSDLQRHIWA HEGVKPFKCS
901LCEYATRSKS NLKAHMNRHS TEKTHLCDMC GKKFKSKGTL KSHKLLHTAD GKQFKCTVCD
961YTAAQKPQLL RHMEQHVSFK PFRCAHCHYS CNISGSLKRH YNRKHPNEEY ANVGTGELAA
1021EVLIQQGGLK CPVCSFVYGT KWEFNRHLKN KHGLKVVEID GDPKWEVTEE EPSSNHTVMI
1081QETVQQASVE LAEQHHLVVS SDDVEGIETV TVYTQGGEAS EFIVYVQEAM QPVEEQAVEQ
1141PAQEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1145 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.