LSBio
ZDHHC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZDHHC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZDHHC8 antibodies →
$725.00each
Catalog Number: LS-G130225-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZDHHC8 (NM_001185024), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHHC-8 | KIAA1292 | ZDHHCL1 | Zinc finger protein 378 | ZNF378 | ZDHHC8 | DHHC8
Origin Species
Human
NCBI GenBank
NM_013373
SwissProt
Q9ULC8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRSPGTRLK PAKYIPVATA AALLVGSSTL FFVFTCPWLT RAVSPAVPVY NGIIFLFVLA
61NFSMATFMDP GVFPRADEDE DKEDDFRAPL YKNVDVRGIQ VRMKWCATCH FYRPPRCSHC
121SVCDNCVEDF DHHCPWVNNC IGRRNYRYFF LFLLSLSAHM VGVVAFGLVY VLNHAEGLGA
181AHTTITMAVM CVAGLFFIPV IGLTGFHVVL VTRGRTTNEQ VTGKFRGGVN PFTRGCCGNV
241EHVLCSPLAP RYVVEPPRLP LAVSLKPPFL RPELLDRAAP LKVKLSDNGL KAGLGRSKSK
301GSLDRLDEKP LDLGPPLPPK IEAGTFSSDL QTPRPGSAES ALSVQRTSPP TPAMYKFRPA
361FPTGPKVPFC GPGEQVPGPD SLTLGDDSIR SLDFVSEPSL DLPDYGPGGL HAAYPPSPPL
421SASDAFSGAL RSLSLKASSR RGGDHVALQP LRSEGGPPTP HRSIFAPHAL PNRNGSLSYD
481SLLNPGSPGG HACPAHPAVG VAGYHSPYLH PGATGDPPRP LPRSFSPVLG PRPREPSPVR
541YDNLSRTIMA SIQERKDREE RERLLRSQAD SLFGDSGVYD APSSYSLQQA SVLSEGPRGP
601ALRYGSRDDL VAGPGFGGAR NPALQTSLSS LSSSVSRAPR TSSSSLQADQ ASSNAPGPRP
661SSGSHRSPAR QGLPSPPGTP HSPSYAGPKA VAFIHTDLPE PPPSLTVQRG RIGTCTRGWG
721RRGQPWVPPG LHLCHLGRPE DRPPLRAPWS QAAGAPPRGA MCRLHLAASS LFPSLSGP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

778 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.