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ZDHHC23 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZDHHC23 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZDHHC23 antibodies →
$504.00each
Catalog Number: LS-G105218-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZDHHC23 (NM_173570), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHHC-23 | NIDD | ZDHHC23
Origin Species
Human
SwissProt
Q8IYP9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
45.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MTQKGSMKPV KKKKTEEPEL EPLCCCEYID RNGEKNHVAT CLCDCQDLDE GCDRWITCKS
61LQPETCERIM DTISDRLRIP WLRGAKKVNI SIIPPLVLLP VFLHVASWHF LLGVVVLTSL
121PVLALWYYYL TNRRKEQTLF FLSLGLFSLG YMYYVFLQEV VPKGRVGPVQ LAVLTCGLFL
181ILLALHRAKK NPGYLSNPAS GDRSLSSSQL ECLSRKGQEK AKGFPGADMS GSLNNRTTKD
241DPKGSSKMPA GSPTKAKEDW CAKCQLVRPA RAWRCRICGI CVRRMDHHCV WINSCVGESN
301HQAFILALLI FLLTSVYGIT LTLDTICRDR SVFTALFYCP GVYANYSSAL SFTCVWYSVI
361ITAGMAYIFL IQLINISYNV TEREVQQALR QKTGRRLLCG LIVDTGLLG
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

409 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.