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ZDHHC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZDHHC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZDHHC14 antibodies →
$725.00each
Catalog Number: LS-G109799-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZDHHC14 (NM_024630), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DHHC-14 | NEW1 domain-containing protein | NEW1 domain containing protein | NEW1CP | ZDHHC14
Origin Species
Human
NCBI GenBank
NM_024630
SwissProt
Q8IZN3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MPPGGGGPMK DCEYSQISTH SSSPMESPHK KKKIAARRKW EVFPGRNKFF CNGRIMMARQ
61TGVFYLTLVL ILVTSGLFFA FDCPYLAVKI TPAIPAVAGI LFFFVMGTLL RTSFSDPGVL
121PRATPDEAAD LERQIDIANG TSSGGYRPPP RTKEVIINGQ TVKLKYCFTC KIFRPPRASH
181CSLCDNCVER FDHHCPWVGN CVGKRNYRFF YMFILSLSFL TVFIFAFVIT HVILRSQQTG
241FLNALKDSPA SVLEAVVCFF SVWSIVGLSG FHTYLISSNQ TTNEDIKGSW SNKRGKENYN
301PYSYGNIFTN CCVALCGPIS PSLIDRRGYI QPDTPQPAAP SNGITMYGAT QSQSDMCDQD
361QCIQSTKFVL QAAATPLLQS EPSLTSDELH LPGKPGLGTP CASLTLGPPT PPASMPNLAE
421ATLADVMPRK DEHMGHQFLT PDEAPSPPRL LAAGSPLAHS RTMHVLGLAS QDSLHEDSVR
481GLVKLSSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

488 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.