Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZDHHC13 / HIP14L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111480-100
Catalog Number: LS-G111480
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZDHHC13 (NM_019028), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DHHC-13 | HIP14-related protein | HIP14L | Palmitoyltransferase ZDHHC13 | ZDHHC13 | HIP3RP
- Origin Species
- Human
- NCBI GenBank
- NM_019028
- SwissProt
- Q8IUH4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGPGLGSQC RNHSHGPHPP GFGRYGICAH ENKELANARE ALPLIEDSSN CDIVKATQYG61IFERCKELVE AGYDVRQPDK ENVSLLHWAA INNRLDLVRF YISKGAVVDQ LGGDLNSTPL121HWAIRQGHLP MVILLLQHGA DPTLIDGEGF SSIHLAVLFQ HMPIIAYLIS KGQSVNMTDV181NGQTPLMLSA HKVIGPEPTG FLLKFNPSLN VVDKIHQNTP LHWAVAAGNV NAVDKLLEAG241SSLDIQNVKG ETPLDMALQN KNQLIIHMLK TEAKMRANQK FRLWRWLQKC ELFLLLMLSV301ITMWAIGYIL DFNSDSWLLK GCLLVTLFFL TSLFPRFLVG YKNLVYLPTA FLLSSVFWIF361MTWFILFFPD LAGAPFYFSF IFSIVAFLYF FYKTWATDPG FTKASEEEKK VNIITLAETG421SLDFRTFCTS CLIRKPLRSL HCHVCNCCVA RYDQHCLWTG RCIGFGNHHY YIFFLFFLSM481VCGWIIYGSF IYLSSHCATT FKEDGLWTYL NQIVACSPWV LYILMLATFH FSWSTFLLLN541QLFQIAFLGL TSHERISLQK QSKHMKQTLS LRKTPYNLGF MQNLADFFQC GCFGLVKPCV601VDWTSQYTMV FHPAREKVLR SVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
622 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.