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ZCWPW1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZCWPW1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112068-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZCWPW1 (NM_017984), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZCW1 | ZCWPW1
Origin Species
Human
NCBI GenBank
NM_017984
SwissProt
Q9H0M4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMTTLQNKEE CGKGPKRIFA PPAQKSYSLL PCSPNSPKEE TPGISSPETE ARISLPKASL
61KKKEEKATMK NVPSREQEKK RKAQINKQAE KKEKEKSSLT NAEFEEIVQI VLQKSLQECL
121GMGSGLDFAE TSCAQPVVST QSDKEPGITA SATDTDNANG EEVPHTQEIS VSWEGEAAPE
181IRTSKLGQPD PAPSKKKSNR LTLSKRKKEA HEKVEKTQGG HEHRQEDRLK KTVQDHSQIR
241DQQKGEISGF GQCLVWVQCS FPNCGKWRRL CGNIDPSVLP DNWSCDQNTD VQYNRCDIPE
301ETWTGLESDV AYASYIPGSI IWAKQYGYPW WPGMIESDPD LGEYFLFTSH LDSLPSKYHV
361TFFGETVSRA WIPVNMLKNF QELSLELSVM KKRRNDCSQK LGVALMMAQE AEQISIQERV
421NLFGFWSRFN GSNSNGERKD LQLSGLNSPG SCLEKKEKEE ELEKEEGEKT DPILPIRKRV
481KIQTQKTKPR GLGGDAGTAD GRGRTLQRKI MKRSLGRKST APPAPRMGRK EGQGNSDSDQ
541PGPKKKFKAP QSKALAASFS EGKEVRTVPK NLGLSACKGA CPSSAKEEPR HREPLTQEAG
601SVPLEDEASS DLDLEQLMED VGRELGQSGE LQHSNSDGED FPVALFGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

648 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.