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ZCCHC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZCCHC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZCCHC8 antibodies →
$504.00each
Catalog Number: LS-G112347-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZCCHC8 (NM_017612), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZCCHC8
Origin Species
Human
NCBI GenBank
NM_017612
SwissProt
Q6NZY4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAEVYFGDL ELFEPFDHPE ESIPKPVHTR FKDDDGDEED ENGVGDAELR ERLRQCEETI
61EQLRAENQEL KRKLNILTRP SGILVNDTKL DGPILQILFM NNAISKQYHQ EIEEFVSNLV
121KRFEEQQKND VEKTSFNLLP QPSSIVLEED HKVEESCAIK NNKEAFSVVG SVLYFTNFCL
181DKLGQPLLNE NPQLSEGWEI PKYHQVFSHI VSLEGQEIQV KAKRPKPHCF NCGSEEHQMK
241DCPMPRNAAR ISEKRKEYMD ACGEANNQNF QQRYHAEEVE ERFGRFKPGV ISEELQDALG
301VTDKSLPPFI YRMRQLGYPP GWLKEVELEN SGLALYDGKD GTDGETEVGE IQQNKSVTYD
361LSKLVNYPGF NISTPRGIPD EWRIFGSIPM QACQQKDVFA NYLTSNFQAP GVKSGNKRSS
421SHSSPGSPKK QKNESNSAGS PADMELDSDM EVPHGSQSSE SFQFQPPLPP DTPPLPRGTP
481PPVFTPPLPK GTPPLTPSDS PQTRTASGAV DEDALTLEEL EEQQRRIWAA LEQAESVNSD
541SDVPVDTPLT GNSVASSPCP NELDLPVPEG KTSEKQTLDE PEVPEIFTKK SEAGHASSPD
601SEVTSLCQKE KAELAPVNTE GALLDNGSVV PNCDISNGGS QKLFPADTSP STATKIHSPI
661PDMSKFATGI TPFEFENMAE STGMYLRIRS LLKNSPRNQQ KNKKASE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

707 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.