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ZCCHC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZCCHC7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZCCHC7 antibodies →
$504.00each
Catalog Number: LS-G108976-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZCCHC7 (NM_032226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIR1 | HSPC086 | RP11-397D12.1 | ZCCHC7
Origin Species
Human
NCBI GenBank
AK026264
SwissProt
Q8N3Z6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGGYETIEA YEDDLYRDES SSELSVDSEV EFQLYSQIHY AQDLDDVIRE EEHEEKNSGN
61SESSSSKPNQ KKLIVLSDSE VIQLSDGSEV ITLSDEDSIY RCKGKNVRVQ AQENAHGLSS
121SLQSNELVDK KCKSDIEKPK SEERSGVIRE VMIIEVSSSE EEESTISEGD NVESWMLLGC
181EVDDKDDDIL LNLVGCENSV TEGEDGINWS ISDKDIEAQI ANNRTPGRWT QRYYSANKNI
241ICRNCDKRGH LSKNCPLPRK VRRCFLCSRR GHLLYSCPAP LCEYCPVPKM LDHSCLFRHS
301WDKQCDRCHM LGHYTDACTE IWRQYHLTTK PGPPKKPKTP SRPSALAYCY HCAQKGHYGH
361ECPEREVYDP SPVSPFICYY DDKYEIQERE KRLKQKIKVL KKNGVIPEPS KLPYIKAANE
421NPHHDIRKGR ASWKSNRWPQ ENKETQKEMK NKNRNWEKHR KADRHREVDE DFPRGPKTYS
481SPGSFKTQKP SKPFHRSSHY HTSREDKSPK EGKRGKQKKK ERCWEDDDND NLFLIKQRKK
541KS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.