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ZCCHC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZCCHC4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZCCHC4 antibodies →
$725.00each
Catalog Number: LS-G109661-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZCCHC4 (NM_024936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSPC052 | ZCCHC4
Origin Species
Human
NCBI GenBank
BC016914
SwissProt
Q9H5U6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASRNGFEA VEAEGSAGCR GSSGMEVVLP LDPAVPAPLC PHGPTLLFVK VTQGKEETRR
61FYACSACRDR KDCNFFQWED EKLSGARLAA REAHNRRCQP PLSRTQCVER YLKFIELPLT
121QRKFCQTCQQ LLLPDDWGQH SEHQVLGNVS ITQLRRPSQL LYPLENKKTN AQYLFADRSC
181QFLVDLLSAL GFRRVLCVGT PRLHELIKLT ASGDKKSNIK SLLLDIDFRY SQFYMEDSFC
241HYNMFNHHFF DGKTALEVCR AFLQEDKGEG IIMVTDPPFG GLVEPLAITF KKLIAMWKEG
301QSQDDSHKEL PIFWIFPYFF ESRICQFFPS FQMLDYQVDY DNHALYKHGK TGRKQSPVRI
361FTNIPPNKII LPTEEGYRFC SPCQRYVSLE NQHCELCNSC TSKDGRKWNH CFLCKKCVKP
421SWIHCSICNH CAVPDHSCEG PKHGCFICGE LDHKRSTCPN IATSKRANKA VRKQKQRKSN
481KMKMETTKGQ SMNHTSATRR KKRRERAHQY LGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

513 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.