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ZCCHC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZCCHC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZCCHC14 antibodies →
$504.00each
Catalog Number: LS-G113410-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZCCHC14 (NM_015144), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BDG-29 | ZCCHC14 | BDG29 | KIAA0579
Origin Species
Human
NCBI GenBank
NM_015144
SwissProt
Q8WYQ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASNHPAFSF HQKQVLRQEL TQIQSSLNGG GGHGGKGAPG PGGALPTCPA CHKITPRTEA
61PVSSVSNSLE NALHTSAHST EESLPKRPLG KHSKVSVEKI DLKGLSHTKN DRNVECSFEV
121LWSDSSITSV TKSSSEVTEF ISKLCQLYPE ENLEKLIPCL AGPDAFYVER NHVDLDSGLR
181YLASLPSHVL KNDHVRRFLS TSSPPQQLQS PSPGNPSLSK VGTVMGVSGR PVCGVAGIPS
241SQSGAQHHGQ HPAGSAAPLP HCSHAGSAGS ALAYRTQMDT SPAILMPSSL QTPQTQEQNG
301ILDWLRKLRL HKYYPVFKQL SMEKFLSLTE EDLNKFESLT MGAKKKLKTQ LELEKEKSER
361RCLNPSAPPL VTSSGVARVP PTSHVGPVQS GRGSHAAELR VEVEQPHHQL PREGSSSEYS
421SSSSSPMGVQ AREESSDSAE ENDRRVEIHL ESSDKEKPVM LLNHFTSSSA RPTAQVLPVQ
481NEASSNPSGH HPLPPQMLSA ASHITPIRML NSVHKPERGS ADMKLLSSSV HSLLSLEERN
541KGSGPRSSMK VDKSFGSAMM DVLPASAPHQ PVQVLSGLSE SSSMSPTVSF GPRTKVVHAS
601TLDRVLKTAQ QPALVVETST AATGTPSTVL HAARPPIKLL LSSSVPADSA ISGQTSCPNN
661VQISVPPAII NPRTALYTAN TKVAFSAMSS MPMGPLQGGF CANSNTASPS SHPSTSFANM
721ATLPSCPAPS SSPALSSVPE SSFYSSSGGG GSTGNIPASN PNHHHHHHHQ QPPAPPQPAP
781PPPGCIVCTS CGCSGSCGSS GLTVSYANYF QHPFSGPSVF TFPFLPFSPM CSSGYVSAQQ
841YGGGSTFPVV HAPYSSSGTP DPVLSGQSTF AVPPMQNFMA GTAGVYQTQG LVGSSNGSSH
901KKSGNLSCYN CGATGHRAQD CKQPSMDFNR PGTFRLKYAP PAESLDSTD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

949 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.