Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZC3HC1 / NIPA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112647-100
Catalog Number: LS-G112647
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NIPA(ZC3HC1) (NM_016478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSPC216 | HNIPA | Zinc finger | C3HC type 1 | ZC3HC1 | NIPA
- Origin Species
- Human
- NCBI GenBank
- NM_016478
- SwissProt
- Q86WB0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPCEGQAF AVGVEKNWGA VVRSPEGTPQ KIRQLIDEGI APEEGGVDAK DTSATSQSVN61GSPQAEQPSL ESTSKEAFFS RVETFSSLKW AGKPFELSPL VCAKYGWVTV ECDMLKCSSC121QAFLCASLQP AFDFDRYKQR CAELKKALCT AHEKFCFWPD SPSPDRFGML PLDEPAILVS181EFLDRFQSLC HLDLQLPSLR PEDLKTMCLT EDKISLLLHL LEDELDHRTD ERKTTIKLGS241DIQVHVTACI LSVCGWACSS SLESMQLSLI ACSQCMRKVG LWGFQQIESS MTDLDASFGL301TSSPIPGLEG RPERLPLVPE SPRRMMTRSQ DATFSPGSEQ AEKSPGPIVS RTRSWDSSSP361VDHPEPEAAS PTTRTRPVTR SMGTGDTPGL EVPSSPLRKA KRARLCSSSS SDTSSRSFFD421PTSQHRDWCP WVNITLGKES RENGGTEPDA SAPAEPGWKA VLTILLAHKQ SSQPAETDSM481SLSEKSRKVF RIFRQWESLC SCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
502 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.