LSBio
ZC3H7B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZC3H7B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112355-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZC3H7B (NM_017590), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RoXaN | ZC3H7B | KIAA1031
Origin Species
Human
NCBI GenBank
NM_017590
SwissProt
Q9UGR2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MERQKRKADI EKGLQFIQST LPLKQEEYEA FLLKLVQNLF AEGNDLFREK DYKQALVQYM
61EGLNVADYAA SDQVALPREL LCKLHVNRAA CYFTMGLYEK ALEDSEKALG LDSESIRALF
121RKARALNELG RHKEAYECSS RCSLALPHDE SVTQLGQELA QKLGLRVRKA YKRPQELETF
181SLLSNGTAAG VADQGTSNGL GSIDDIETDC YVDPRGSPAL LPSTPTMPLF PHVLDLLAPL
241DSSRTLPSTD SLDDFSDGDV FGPELDTLLD SLSLVQGGLS GSGVPSELPQ LIPVFPGGTP
301LLPPVVGGSI PVSSPLPPAS FGLVMDPSKK LAASVLDALD PPGPTLDPLD LLPYSETRLD
361ALDSFGSTRG SLDKPDSFME ETNSQDHRPP SGAQKPAPSP EPCMPNTALL IKNPLAATHE
421FKQACQLCYP KTGPRAGDYT YREGLEHKCK RDILLGRLRS SEDQTWKRIR PRPTKTSFVG
481SYYLCKDMIN KQDCKYGDNC TFAYHQEEID VWTEERKGTL NRDLLFDPLG GVKRGSLTIA
541KLLKEHQGIF TFLCEICFDS KPRIISKGTK DSPSVCSNLA AKHSFYNNKC LVHIVRSTSL
601KYSKIRQFQE HFQFDVCRHE VRYGCLREDS CHFAHSFIEL KVWLLQQYSG MTHEDIVQES
661KKYWQQMEAH AGKASSSMGA PRTHGPSTFD LQMKFVCGQC WRNGQVVEPD KDLKYCSAKA
721RHCWTKERRV LLVMSKAKRK WVSVRPLPSI RNFPQQYDLC IHAQNGRKCQ YVGNCSFAHS
781PEERDMWTFM KENKILDMQQ TYDMWLKKHN PGKPGEGTPI SSREGEKQIQ MPTDYADIMM
841GYHCWLCGKN SNSKKQWQQH IQSEKHKEKV FTSDSDASGW AFRFPMGEFR LCDRLQKGKA
901CPDGDKCRCA HGQEELNEWL DRREVLKQKL AKARKDMLLC PRDDDFGKYN FLLQEDGDLA
961GATPEAPAAA ATATTGE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

977 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.