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ZC3H14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZC3H14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZC3H14 antibodies →
$725.00each
Catalog Number: LS-G128334-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZC3H14 (NM_001160104), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NY-REN-37 | Nuclear protein UKp68 | SUT2 | MSUT-2 | UKp68 | ZC3H14
Origin Species
Human
NCBI GenBank
NM_024824
SwissProt
Q6PJT7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEIGTEISRK IRSAIKGKLQ ELGAYVDEEL PDYIMVMVAN KKSQDQMTED LSLFLGNNTI
61RFTVWLHGVL DKLRSVTTEP SSLKSSDTNI FDSNVPSNKS NFSRGDERRH EAAVPPLAIP
121SARPEKRDSR VSTSSQESKT TNVRQTYDDG AATRLMSTVK PLREPAPSED VIDIKPEPDD
181LIDEDLNFVQ ENPLSQKKPT VTLTYGSSRP SIEIYRPPAS RNADSGVHLN RLQFQQQQNS
241IHAAKQLDMQ SSWVYETGRL CEPEVLNSLE ETYSPFFRNN SEKMSMEDEN FRKRKLPVVS
301SVVKVKKFNH DGEEEEEDDD YGSRTGSISS SVSVPAKPER RPSLPPSKQA NKNLILKAIS
361EAQESVTKTT NYSTVPQKQT LPVAPRTRTS QEELLAEVVQ GQSRTPRISP PIKEEETKGD
421SVEKNQGTQQ RQLLSRLQID PVMAETLQMS QDYYDMESMV HADTRSFILK KPKLSEEVVV
481APNQESGMKT ADSLRVLSGH LMQTRDLVQP DKPASPKFIV TLDGVPSPPG YMSDQEEDMC
541FEGMKPVNQT AASNKGLRGL LHPQQLHLLS RQLEDPNAEM SELSVAQKPE KLLERCKYWP
601ACKNGDECAY HHPISPCKAF PNCKFAEKCL FVHPNCKYDA KCTKPDCPFT HVSRRIPVLS
661PKPVAPPAPP SSSQLCRYFP ACKKMECPFY HPKHCRFNTQ CTRPDCTFYH PTINVPPRHA
721LKWIRPQTSE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

730 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.